Coding

Part:BBa_K4475006:Design

Designed by: Andrew Taylor   Group: iGEM22_FSU   (2022-08-21)


CTB3 Gene from Cercospora beticola


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1263
    Illegal XbaI site found at 100
    Illegal XbaI site found at 457
    Illegal XbaI site found at 925
    Illegal XbaI site found at 1243
    Illegal XbaI site found at 1381
    Illegal XbaI site found at 1651
    Illegal XbaI site found at 2104
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1263
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1263
    Illegal BglII site found at 388
    Illegal BglII site found at 562
    Illegal BglII site found at 1291
    Illegal BglII site found at 2563
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1263
    Illegal XbaI site found at 100
    Illegal XbaI site found at 457
    Illegal XbaI site found at 925
    Illegal XbaI site found at 1243
    Illegal XbaI site found at 1381
    Illegal XbaI site found at 1651
    Illegal XbaI site found at 2104
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1263
    Illegal XbaI site found at 100
    Illegal XbaI site found at 457
    Illegal XbaI site found at 925
    Illegal XbaI site found at 1243
    Illegal XbaI site found at 1381
    Illegal XbaI site found at 1651
    Illegal XbaI site found at 2104
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Cut site eligibility for RFC 1000 achieved through silent mutations. N-terminal 6xHis tagged and codon optimized for yeast.

Source

Genome of Cercospora beticola https://www.ncbi.nlm.nih.gov/gene/35424646

Taken From

de Jonge R, Ebert MK, Huitt-Roehl CR, Pal P, Suttle JC, Spanner RE, Neubauer JD, Jurick WM 2nd, Stott KA, Secor GA, Thomma BPHJ, Van de Peer Y, Townsend CA, Bolton MD. Gene cluster conservation provides insight into cercosporin biosynthesis and extends production to the genus Colletotrichum. Proc Natl Acad Sci U S A. 2018 Jun 12;115(24):E5459-E5466. doi: 10.1073/pnas.1712798115. Epub 2018 May 29. Erratum in: Proc Natl Acad Sci U S A. 2018 Aug 28;115(35):E8324. PMID: 29844193; PMCID: PMC6004482.